PBS vs HBSS - Difference Between

PBS vs HBSS

“PBS” is the abbreviation for phosphate buffered saline while HBSS is the acronym for Hank’s balanced salt solution. These two are forms of a saline solution with added ingredients or chemicals to achieve their purposes.

Both PBS and HBSS are used in cell biology, mostly in molecular biology and biochemistry experiments and overall biological research. During a biological experiment, both solutions are placed in a Petri dish with a cell or tissue. The solution holds the cell or tissue for examining or for use during the activity.

Phosphate buffered saline, as its name implies, is a buffer. It strives to maintain a neutral pH in order not to destroy the cell or tissue sample and maintain the osmolarity of the cells. It is also non-toxic to cells.

As a buffer solution, it is a mixture of a “weaker” base or acid with a corresponding conjugate acid or base. Buffer solutions are usually solutions that are added into another solution for purposes of maintaining a particular level of pH. Aside from performing as a buffer, they are also used to dilute substances and rinse containers that used to hold cells for effective sanitation.

The main ingredients of phosphate buffered solutions are: water, sodium chloride, and sodium phosphate. In some recipes, there is an addition of potassium chloride and potassium phosphate.

Meanwhile, Hank’s balanced salt solution is also an isotonic solution that acts to maintain a level of pH and the sample’s osmotic pressure. It provides the sample cell or tissue with the sufficient amount of salt ions that balances the amount of water inside the cell. This results in preserving the sample in its natural state and structure. Providing the wrong amount of salt ions in the solution can lead to cell shrinkage or bursting.


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Balanced Salt Solution - Bookshelf

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Daily Knowledge Directory


Balanced salt solution - Wikipedia, the free encyclopedia
In medicine, balanced salt solutions can be used as an irrigation solution such as during intraocular surgery and to replace intraocular fluids. ...

Balanced salt solution: Definition from Answers.com
balanced salt solution A sterile solution of sodium chloride , sodium acetate, sodium citrate , calcium chloride , and magnesium chloride, used to

Balanced Salt Solution
Balanced Salt Solution (New-Zealand; England; Denmark; Finland) ... BSS Sterile Irrigating Solution is a sterile physiological balanced salt solution, each ml, containing Sodium ...

Buffered Salt Solutions - Products
is a manufacturer of sterile solutions ranging from cell culture media, molecular biology reagents, bioprocessing containment systems, and custom manufacturing. ...

Balanced Salt Solutions
Balanced Salt Solutions. Part Number. Component Description. mg/L. mmol/L ... Balanced Salt Solutions. P a rt N um be r. C o m po ne nt D e s c ript ...